| Course Name | Cell Culture and Animal Lab |
| Course Code | 22MMD582 |
| Program | M. Sc. Molecular Medicine(MLM) |
(Lab sessions = 3)
General lay out of a cell culture lab, physical environment needed for the cell culture, growth media and its composition, BSC and its use in cell culture and how to work in a BSC, contamination during cell culture and how to control it, culturing and splitting of cell lines, cryopreservation of cells and cell viability assays.
(Lab sessions = 7)
Hands-on cell culture work, media changes, seeding, splitting adherent cells, cell counting, reseeding, and safe disposal
(Lab sessions = 5)
Animal handling techniques, animal feed, gavage, different routes of injection, ethical treatment of animals and Institutional Animal Ethics Committee policies
Pre-requisites: Basic understanding of biology
Total number of lab sessions: 30
Preamble: Cell culture module introduces the students to the basics of cell culture. The course provides students with sufficient knowledge and laboratory skills needed in academia and industry for carrying out basic cell culture techniques properly and safely. The animal handling module introduces students to the regulations, ethics and importance of animal use in research, along with basic small animal handling techniques
Course Outcome
CO1: To demonstrate a general level of understanding towards the function, maintenance and working of Bio-safety Cabinets (BSC) and be able to work in BSCs with a good sterilization technique
CO2: To identify culture contamination and methods involved to maintain sterility
CO3: Able to prepare media and maintain adherent cells in culture for atleast a week
CO4: To become aware of standard practices in cell culture and related ethical dilemmas
CO5: Be able to handle small animals and become familiar with ethics involved in animal use for research.
Program Outcomes (PO) (As given by NBA and ABET)
PO1: Bioscience Knowledge
PO2: Problem Analysis
PO3: Design/Development of Solutions
PO4: Conduct Investigations of complex problems
PO5: Modern tools usage
PO6: Bioscientist and Society
PO7: Environment and Sustainability
PO8: Ethics
PO9: Individual & Team work
PO10: Communication
PO11: Project management & Finance
PO12: Lifelong learning
| C/CO | PO1 | PO2 | PO3 | PO4 | PO5 | PO6 | PO7 | PO8 | PO9 | PO10 | PO11 | PO12 |
| CO1 | 3 | 1 | 3 | 3 | 1 | 1 | 2 | |||||
| CO2 | 2 | 3 | 3 | 1 | 1 | 2 | ||||||
| CO3 | 2 | 3 | 3 | 3 | 3 | 3 | 1 | 2 | ||||
| CO4 | 2 | 1 | 3 | 3 | 3 | 3 | 2 | 2 | ||||
| CO5 | 2 | 1 | 2 | 3 | 3 | 3 | 3 | 2 | 2 |
Program Specific Outcomes (PSO)
PSO 1 – Biochemical organization and cellular complexity in function
PSO 2 – Biomolecules in Medicine
PSO 3 – Molecular dysregulation in diseases
PSO 4 – Molecular technology in diagnosis and therapy
PSO 5 – Cell based approaches in diagnosis and therapy
PSO 6 – Microorganisms in Medicine
PSO 7 – Nanoscale entities and its significance in Medicine
PSO 8 – Tissue architecture engineering in Medicine
PSO 9 – Compounds as drugs and its efficacy
PSO 10 – Bioinformatics and biological data use
| C/CO | PSO1 | PSO2 | PSO3 | PSO4 | PSO5 | PSO6 | PSO7 | PSO8 | PSO9 | PSO10 |
| CO1 | 1 | 1 | 3 | 1 | ||||||
| CO2 | 3 | 1 | ||||||||
| CO3 | 3 | 2 | ||||||||
| CO4 | 3 | 1 | ||||||||
| CO5 | 3 | 2 |
Evaluation Pattern: 30+70 = 100
| Internal Assessment – 30% | ||
| Records | Evaluation | 30% |
| 30% | ||
| End Semester Examination- 50% | ||
| Practical | Exam | 60% |
| Assessment | Viva | 10% |
| 70% | ||
| Total | 100% | |
Reference
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