Project

Production, Purification and Application of Naringinase from soil isolates of Aspergillus spp

Aspergillus niger capable of hydrolyzing naringin by growing the fungi at 280 C, on selective synthetic minimal medium, pH 6, containing 1% naringin was selected for the study. Naringinase enzyme was produced using the production media and the enzyme was precipitated using 60% ammonium sulphate. Purification was done using anion exchange chromatography. Enzyme was characterized in respect to pH and temperature. Application study revealed good results. There was an increase in enzyme activity in the presence of Mg+.